Bulb scales were cultured on solidified (0.1% Phytogel and 0.4% Junsei agar) MS media
supplemented with 0.0–22.2 M BA to induce shoots from base part of bulb scales.
In order to proliferate shoots, shoot clusters were cut longitudinally into 5–7mm segments,
and cultured on media containing BA (0.0–22.2 M) and IAA (0.0–5.7 M).
Shoots were multiplicated by shoot cluster segment–shoot cluster cycle method on MS medium with
30 g/L sucrose, and 2.2 M BA and 2.9 M IAA.