The esp1 mutant CM21 specifically exhibits reduced levels of cysteine-poor (CysP) prolamin bands with
pIs of 6.65, 6.95, 7.10, and 7.35 in rice seed. Matrix-assisted laser desorption-ionization time-of-flight
mass spectrometry (MALDI-TOF MS) analysis demonstrated that the bands with pIs 6.65, 6.95, and 7.35
are encoded by different structural genes. These results suggest that the Esp1 locus encodes a regulatory
factor involved in the synthesis and/or accumulation of CysP prolamin molecules. Isoelectric focusing (IEF)
analysis of CysP prolamins in chromosome substitution lines showed that structural genes for bands with
pI values of 6.95, 7.10, and 7.35, which are reduced in esp1 mutant lines, are located as a gene cluster in
the 44.2 cM region on chromosome 5.
The esp1 mutant CM21 specifically exhibits reduced levels of cysteine-poor (CysP) prolamin bands withpIs of 6.65, 6.95, 7.10, and 7.35 in rice seed. Matrix-assisted laser desorption-ionization time-of-flightmass spectrometry (MALDI-TOF MS) analysis demonstrated that the bands with pIs 6.65, 6.95, and 7.35are encoded by different structural genes. These results suggest that the Esp1 locus encodes a regulatoryfactor involved in the synthesis and/or accumulation of CysP prolamin molecules. Isoelectric focusing (IEF)analysis of CysP prolamins in chromosome substitution lines showed that structural genes for bands withpI values of 6.95, 7.10, and 7.35, which are reduced in esp1 mutant lines, are located as a gene cluster inthe 44.2 cM region on chromosome 5.
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