Shoot proliferation was proportionally enhanced by increasing concentrations of BA, as shown in Table 1, and in vitro shoot morphology was greatly affected by BA concentration. Several reductions in the number shoot per explant were observed at the concentration of BA (12.0 μM). Therefore, this BA concentration harmed B. vulgaris shoot length and number of leaves per shoot proliferation in static liquid culture medium. Similar re- sults, have been described in Bambusa tulda, Bambusa atra and Dendrocalamus giganteus [28] and Dendrocalamus hookeri [29]. However, the number shoots per explant were increased with 6.0 μM BA in the TIS. The positive effects of TIS on the nutrient assimilation have been demonstrated during the growth and development of bamboo [30