2.3. Phylogenetic analysis of endophytic fungi
For strain identification, the ITS sequences were compared with those in the NCBI database (http://www.ncbi.nlm.nih.gov/) using the BLAST search program for the final identification of the fungal endophytes program. A phylogenetic tree was constructed by the neighbor-joining method using MEGA version 5.0 software (http://www.megasoftware.net/), after pairwise alignments using the CLUSTAL X program, version 1.8 (http://www.clustal.org/) [33]. The stability of the internal branches was assessed with 1,000 bootstrap replications. The sequences of this study were deposited in the GenBank database under the accession numbers: KP714353–KP714393 (Table 1).