After sterilization in the bag, the bag is the inoculation of pure strains of the pre-vaccination or vaccination of the grain to realize. In order to reduce development time, rocking the bag in order to make the strain or pre-vaccination of the grain and cereal mix. Furthermore, in about 80 °F cultivating the bag at a temperature of approximately 3 weeks. During this period, most of the digested bacterial strain (if not all, of the words) matrix in order to produce the mycelium.