2.2. Batch Fermentation Condition
The modied terric broth with optimized formulation
(Terric Broth [Himedia, Mumbai, India] containing 8 mL/L
of glycerol, 2X-M9 salts [Difco], 2X Yeast extract, 1.2 mL/L
of MgCl2, and 1 mL/L trace metal solution) was found an
optimum medium composition for the expression of
rBimA protein at shake ask level (data not shown) and
nally was selected for culturing the cells in the batch
fermentation.