.
2.3. Data collection and analysis
Visual observations were made every day. Total fresh weight of protocorm-like bodies was recorded at 0 and after 3, 6, 8 and 12 weeks. The number of plantlets generated by the protocorm-like bodies maintained on agar medium were counted after 12 weeks of cultivation. Three replicates were carriedoutineachexperiment.AlldatawereanalyzedbySPSS software, version 7.2 using one-way ANOVA analysis. All photographs are representative for the growth condition as described.