UV-visible and fluorescence spectroscopy determination
of two types of DNA sequences bound
to gold nanoparticles
The number of strands of each DNA per AuNP was determined
by dividing the concentration of each DNA sequence
by the concentration of AuNPs. The concentration of DNANPs
was determined as described previously. The concentration
of the longer DNA strand was determined using the Oligreen
dye assay and used to calculate the number of DNA
strands per AuNP. The extinction coefficient of the longer
DNA strand was then used to determine its contribution to
A260 decomposed DNA-NPs. The absorbance of the shorter
DNA strand was then calculated by subtracting the contributions
from the decomposed nanoparticles and the longer DNA
strand from A260 decomposed DNA-NPs, and using the
DNA’s extinction coefficient to calculate its concentration.
The percentage of each strand in the ligand shell was then
calculated.