All PCR reaction mixtures were overlaid
with 8 µl mineral oil. In order to visualize possible selfpoly merization
or DNA contamination, a negative control
PCR reaction with sterile distilled water in place of
genomic DNA was added to the PCR series. The stability
of PCR conditions was checked using a positive PCR
reaction with a known DNA template and the specific
forward and reverse primers producing a 620 bp product.
Two independent PCR reactions were conducted for all
amplifications reactions.