Leaf explants were cultured on media containing 0.005–50 mol NAA together with 4.5mol BA and the occurrence of variants in the regenerated plants was examined. Leaf segments were also cultured on growth regulator-free medium and examined for
the occurrence of variants. All multiple shoots initiated were sub-cultured as described above.
Explant sources were excised from 10 stock plants. Ten tubes (replication) were selected from each stock plants. The colour patterns of the leaves from five or six regenerated plants were examined from one tube.