Effect of Fermentation Time on Plant Costs and Throughput.
Initial simulations were conducted for the base-case
scenario (Figure 2), considering only one equipment per
stage, adopting starch medium for inoculum propagation
and assuming that the concentrated enzyme stream is
the only revenue source (fermented cake being a waste).
Therefore, streams 17, 18, and 20 in Figure 2 were treated
as waste streams, with a treatment cost for disposal of
0.0005 USD kg−1, for each stream. All solid streams were
transported using belt conveyors. Centrifugal pumps used
for transporting liquid streams are not shown in the