A dendrogram was generated by SM coefficient.
The similarity degree (SD) value for the isolates ranged wide and diverse genetic base. A repeat sequence termed
MGR586 was identified in the genome of rice infecting
strains of M. grisea7. This sequence has been widely used
for DNA fingerprinting of M. grisea to investigate the
epidemiology of the rice blast disease8–11. Another retrotransposon,
fosbury has also been used for genetic differentiation
studies7 and the results indicate that isolates
from Bangladesh lack both MGR586 and fosbury.
MGR586 probe also failed to detect karyotypic changes19.
Thus there is a need to develop different DNA fingerprinting
techniques to identify various forms of M. grisea
diversity. RAPD markers used in this investigation increase
the marker density for finding out genetic relationships.