frozen for up to 3 months have been used in the IVP test,
with IVP rate inferior to that observed with fresh oocytes
matured in vitro (Tatemoto et al., 1994), which was observed
in the present study only with incubation in BTS. The use of
cooled oocytes may contribute to simplify the IVP test, since
it would not need to be executed immediately after oocytes
harvesting. Vitrified oocytes may be used for the IVP