Implantation of electromyographic electrode and histochemical staining of recording site. This series of micrographs is taken from the same fish.
(A) The dilator operculi muscles (DOb and DOg) are exposed after removing the overlying scales. OP, operculum. Inset: electromyographic electrode
made of two Teflon-coated stainless steel wires twisted together. About 0.4 mm of the Teflon coat at the tip of both wires has been stripped off to expose
the metal (arrows). Scale bar 0.5 mm. (B) A pair of electromyographic recording wires has been inserted into DOg. The electrode is glued to the top of the
head with acrylic tissue adhesive (arrow). Inset: a higher magnification of the marked area showing the electrode in the muscle (arrow). Scale bar in inset
0.5 mm. (C) Scales are reattached with the aid of tissue adhesive to cover muscle and electrode (arrow). (D) After EMG recording, iron was deposited at
the recording site by passing current through the EMG electrode. Potassium ferricyanide staining reveals the iron deposit at the recording site in DOg.
Inset: a higher magnification of the marked area showing that iron deposits are confined precisely to the site where the electrode was implanted (see inset in
(B)). Scale bar 0.5 mm. Anterior is to the left in all panels. Scale bar in (C) applies to all panels