2.2. SSR analysis
Microsatellite loci were developed by restriction-site-associated DNA (RAD) sequencing using Illumina HiSeq2000 sequencer (Inc., San Diego, California, USA). Primers were designed for these sequences using the program Primer3 (Rozen and Skaletsky, 2000) embedded in Msatcommander. These primers were tagged with the universal M13 sequence (GTAAAACGACGGCCAGT).