1. The optimum conditions for lyophilized silver-line grunt skin collagen extraction and purification were soaking in 0.1N NaOH for 4 h, washing with distilled water, and suspending in 0.5M acetic acid at a ratio of skin to 0.5M acetic acid of 1:70 for 24 h. Extractions were done three times, to give high yields of 90.4+5.3%. In order to remove telopeptide, 0.1% pepsin with 6 h digestion was used.