Isolation of endophytes is a critical step, because it requires sensitivity to recover a maximum number of colonized endophytes and should be accurate enough to eliminate the epiphytic microbes which are present on the plant surface. Endophytes can be isolated from various plant parts such as seeds, leaves and stems. The collected plants for studying endophytic communities should look apparently healthy and disease free plant, i.e. they do not display any visual symptoms of diseases, in order to minimize the presence of plant pathogenic and saprobic species, and to prevent the isolation of localized pathogenic endophytic microorganisms (Strobel 2003, Strobel & Daisy 2003).