A 50 lL anthocyanin extract was mixed with same volume
of 6 N HCl in a sealed HPLC vial. The mixture was heated in boiling
water bath for 2 h after thorough vortexing. The sample was cooled
in ice bath and dried in an Eppendorf Vacufuge before re-constituted
in 500 lL of water acidified with 5% formic acid. Mass spectrometric
scanning was perform