4). Therefore, it is evident from the present study that not
only biochemical engineering (nutrient stress) but the metabolic
engineering of fungus using suitable gene together with bioprocess
optimization could be an effective combined strategy to increase
the storage lipid productivity. Critical analysis of our experimental
data (Table 3) reveals that the lipid content in the transformed
Colletotrichum is ,1.9-fold higher under standard growth
condition, and ,2.2-fold more after model-based optimization
of culture conditions as compared to the wild type strain grown at
standard condition. In a previous report, the fungal genes coding
for mailc enzyme from Mucor circinelloides (malEMt) and
Mortierella alpine (malEMc) were overexpressed