shows the results of the mullet erythrocyte
experiments at 37 ◦C. In this experiment no differences
were observed between the different exposure times
and it was impossible to assess the 6 and 24 h exposure
times because no viable cells survived at any of the
MMS concentrations nor in the control groups without
MMS. In addition, at this temperature almost all the
cells (>97%) showed DNA damage irrespective of the
MMS concentration to which they had been exposed.
The damage index value for the control at 2 h was statistically
higher than at 1 h. When compared with thecontrols, DNA damage values were significantly increased
at all MMS concentrations with the exception
of the image length values for the 2 × 10−5M MMS
concentration at 2 h exposure time. There was no statistical
differences between the MMS concentrations,
except for the fact that the image length value for the
2 h 8×10−5M MMS concentration group was statistically
higher than for the 2×10−5M and 4×10−5M
MMS groups.