Local brown rice grains (Phetchaburi 1 brown rice) cultivated in Phetchaburi Thailand were
washed in tap water and drained. The grains were soaked in cold clean tap water in the ratio of 1:3
(grain to water) for 6 hrs. After soaking, the grains were placed on moist filter paper in covered glass
dishes and kept at room temperature in the dark for 24 hrs. GBR was dried with a tray dryer at 50oC
until moisture content was less than 13%. The grains were milled (wet-milling process) into flour,
sieved (70 mesh screen) and packaged in labeled air-tight polythene bag for use.