This thesis is create a Microcontroller Laboratory Set for study the performance of the STM32F4 Microcontroller and developmental programming. Using MATLAB with other connected devices. is divided into 3 part : STM32F4 Microcontroller Laboratory Set, Worksheets trial and Manual. The experiment consists of 2 major section is a section of the main control circuit board which is the STM32F4 Microcontroller circuit board and circuit board connections. In The leaves and the trial will be divided into a main and 11 working together, which is a basic 9 leaves.And job applications 2 by the preparation of the trial and to allow students to learn from Basic to applied STM32F4 microcontroller board with the device. Other connections Have knowledge and understanding of the work and development of treatments microcontroller.STM32F4 using MATLAB