2.9. Fruit extraction using a Soxhlet-type apparatus
Powdered fruits (500 g) were extracted using a Soxhlet apparatus with 1.3 l of methanol:water (8:2, v/v) at 55–65 C for 16 h. The extract was filtered through Whatman filter paper (No. 1) to
remove any particles. The extracts were completely concentrated using a rotary evaporator (NN series, EYELA, Tokyo, Japan) in a water bath at 40 C. The fruit concentrate was freeze-dried and
kept at 20 C for anticancer property testing.