Although this method has been used by plant breeders
since the 1960s for biotic resistance development in crop
plants, it is still being used for the purpose. Sometimes,
it is combined with molecular breeding techniques like
marker assisted selection (MAS) in order to make the
selection process easy and effective. Danquah and Barrett
(2002) used restriction fragment length polymorphism
(RFLP) markers to study the powdery mildew resistance
pattern in a barley Cambridge Composite Cross Five
(CCV) population. Some hordein patterns were conserved
in the three populations studied and hordein patterns
showed strong correlation with powdery mildew