For large scale plant production, in vitro regenerated shoot budswere subcultured onto MS medium supplemented with 1.0 mg/lBAP + 0.5 mg/l KIN + 1.0 mg/l GA3 combination. The cultures were subcultured on to the fresh same media composition once in 2weeks interval. This process was repeated for another five subcultures (each 15 days) to examine the effect of subculture onproduction of large scale shoot buds. After 75 days of culture multiple shoots were counted for analysis of total number of regenerated shoot buds.