Sporothrix isolates were collected in Chile in the province of
Vi~na del Mar. Nail samples were obtained by scraping from superficial
and deep layers of the right first toe-nail from a 64-
year-old female patient. Environmental samples (soil) were
taken from different areas of patient’s home garden. Isolations
were made directly on potato dextrose agar (PDA), Sabouraud
dextrose agar (SDA) and Mycosel agar (Cruz Choappa
et al. 2014). Colonies resembling Sporothrix were subcultured
and stored on SDA. A total of 120 reference isolates were
used (Table 1) and obtained from the Federal University of
S~ao Paulo (UNIFESP), S~ao Paulo, Brazil, CBS-KNAW Fungal Biodiversity
Centre, Utrecht, The Netherlands (CBS), and online at
GenBank (http://www.ncbi.nlm.nih.gov/genbank/). The isolates
were of environmental, clinical, or veterinary origins
from different geographic regions of the world. Type strain
for Sporothrix and Ophiostoma species were included in all analyses
when possible.