This study investigated the conversion of Lemna minor biomass to bioethanol. The biomass was
pre-treated by steam explosion (SE, 210 C, 10 min) and then subjected to simultaneous saccharification
and fermentation (SSF) using Cellic CTec 2 (20 U or 0.87 FPU g1 substrate) cellulase plus b-glucosidase
(2 U g1 substrate) and a yeast inoculum of 10% (v/v or 8.0 107 cells mL1
). At a substrate concentration
of 1% (w/v) an ethanol yield of 80% (w/w, theoretical) was achieved. However at a substrate concentration
of 20% (w/v), the ethanol yield was lowered to 18.8% (w/w, theoretical). Yields were considerably
improved by increasing the yeast titre in the inoculum or preconditioning the yeast on steam exploded
liquor. These approaches enhanced the ethanol yield up to 70% (w/w, theoretical) at a substrate concentration
of 20% (w/v) by metabolising fermentation inhibitors.
2015 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license
(htt
This study investigated the conversion of Lemna minor biomass to bioethanol. The biomass waspre-treated by steam explosion (SE, 210 C, 10 min) and then subjected to simultaneous saccharificationand fermentation (SSF) using Cellic CTec 2 (20 U or 0.87 FPU g1 substrate) cellulase plus b-glucosidase(2 U g1 substrate) and a yeast inoculum of 10% (v/v or 8.0 107 cells mL1). At a substrate concentrationof 1% (w/v) an ethanol yield of 80% (w/w, theoretical) was achieved. However at a substrate concentrationof 20% (w/v), the ethanol yield was lowered to 18.8% (w/w, theoretical). Yields were considerablyimproved by increasing the yeast titre in the inoculum or preconditioning the yeast on steam explodedliquor. These approaches enhanced the ethanol yield up to 70% (w/w, theoretical) at a substrate concentrationof 20% (w/v) by metabolising fermentation inhibitors. 2015 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license(htt
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