This crude extract of fruits was
prepared in the following manner: 10 g of each freeze-dried fruit
(FDF)was macerated and dissolved in 100 mL of the ethanol 70 GL.
The mixturewas homogenized using an Ultra Turrax (Polytron, MR-
2100) for three minutes, sonicated for 30 min in an ice bath,
agitated in a 38 C water bath for 60 min at 150 rpm, filtered
(Whatman N1), evaporated to 40 mL, aliquoted and the material
was frozen at 80 C for subsequent analysis. Crude extracts were
prepared in triplicate.
This crude extract of fruits was
prepared in the following manner: 10 g of each freeze-dried fruit
(FDF)was macerated and dissolved in 100 mL of the ethanol 70 GL.
The mixturewas homogenized using an Ultra Turrax (Polytron, MR-
2100) for three minutes, sonicated for 30 min in an ice bath,
agitated in a 38 C water bath for 60 min at 150 rpm, filtered
(Whatman N1), evaporated to 40 mL, aliquoted and the material
was frozen at 80 C for subsequent analysis. Crude extracts were
prepared in triplicate.
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