Lactic acid and sugar concentrations in fermentation samples and coffee pulp hydrolysate were analyzed by high performance liquid chromatography: 10 lL of sample volume was added on a Eurokat H column and eluted isocratically with 0.8 mL min1 of 5mMH2SO4. Detection was carried out by a refractive index detector. Each analysis was carried out in duplicate and peak areas and retention times were compared to analyses of known concentrations of pure lactic acid, glucose, xylose, arabinose and sucrose. The mean concentration of two analyses is presented.