In this work, hemp (Cannabis sativa L.) seed oil was produced by extraction with supercriticalCO2 underdifferent extraction conditions (temperature, pressure and time). The objective was to evaluate the influence of extraction conditions on concentrationof tocopherols, fatty acids and pigments in hemp seed oil. The composition of hemp seed oil obtained with supercritical CO2 was compared with the hemp oilextracted by n-hexane using Soxhlet method and with oil obtained by pressing using screw expeller. Using supercritical CO2 extraction the extracts higher in concentration of tocopherol were produced.The amount of tocopherol in supercritical extracts ranged from 37.09 to 110.61 mg L−1, dependingon the applied process conditions, while-tocopherol content was significantly higher (2–3 times). Thecontent of pigments in the hemp oil obtained by supercritical CO2 had been changed significantly duringthe extraction time from 9.79 to 178.76 mg kg−1 for total chlorophyll content and 8.15 to 57.66 mg kg−1for total carotene content. By selecting the relevant process conditions of supercritical extraction it ispossible to obtain hemp seed