Owen et al. (2010) also analyzed the sensitivity and specificity of the TEMPO® EB system by testing Enterobacteriaceae strains and non-Enterobacteriaceae strains at two inoculum levels (low and high). They concluded that the TEMPO® EB showed to be sensitive and specific.
According to the results, the Petrifilm EB system and the TEMPO® EB system can both be used as a valid alternative to ISO 21528-2:2004 for the Enterobacteriacea assay in milk, based on the good correlation between the results, enhanced ease-of-operation and a reduction in the amount of time customarily required by these systems.