5 g of the sample was weighed into a 250 ml
beaker and 200 ml of 10% acetic acid in ethanol
was added and covered and allowed to stand for 4
h. This was filtered and the extract was
concentrated on a water bath to one-quarter of the
original volume. Concentrated ammonium
hydroxide was added dropwise to the extract until
the precipitation was complete. The whole solution
was allowed to settle and the precipitated was
collected and washed with dilute ammonium
hydroxide and then filtered. The residue is the
alkaloid, which was dried and weighed7