Crude extract of the biosurfactant was recovered from the culture supernatant of D. caeni PO5 by extraction with several organic solvents. Among the four solvent systems, ethyl acetate extraction was the most efficient in terms of biosurfactant revovery from the culture supernatant of D. caeni PO5 (data not shown). A recovery yield of 3.12 g/l was obtained. The use of ethyl acetate resulted in a greater activity of the crude extract when compared with system based on mixtures of choloroform and methanol, cold acetone, or dichloromethane (data not shown). It has also been reported that the extraction of bioproducts with considerably high polarity by ethyl acetate solvent is quite efficient (Saimmai et al. 2013a). Because the recovery and concentration of biosurfactants from fermentation broth largely determines the production cost, our results indicate that ethyl acetate is a better choice than the highly toxic chloro-organic compounds.