‘Papaya ringspot virus’ is one of the major impediments in papaya cultivation. Intergeneric crossing carried out using the ‘PRSV’ resistant wild species Vasconcellea cauliflora as male parent, with the papayavariety ‘Arka Surya’ as female parent resulted in the intergeneric hybrid progenies. The progenies found resistant after artificial inoculation were field planted. The progenies segregated for castor and papayatype leaves with fruits having yellow and pink pulp. The tolerant hermaphrodite progenies selected based on the fruit characteristics were selfed. Five generations of single plant selection was carried out by selfing the hermaphrodite tolerant progenies having castor type leaf. The hybrids in the sixth generation werevalidated using ISSR markers. The banding pattern was obtained with the primers UBC-815, UBC-834,UBC-836, UBC-841 and UBC-844 showed that they are intergeneric hybrids