Effects of reducing mannitol concentration
and treatment with activated charcoal on formation
of C. arabica macrocolonies and embryos
after 8 weeks of incubation. Petri dishes were 6
cm in diameter. Protoplasts (10s
/ml) were plated
on the central circular region (35 mm in diameter)
in the petri dish in modified MS medium
supplemented with 5 µM BA, 0.5 M mannitol,
and 0.3% Gelrite. The rest of the area, which
surrounded the central circle, was filled with
0.9% agar-solidified, modified MS medium
supplemented with 5 µM BA (treatment I); 5 µM
BA plus 0.5 M mannitol (treatment II); 5 µM BA
plus 1% activated charcoal (treatment III); and 5
µM BA plus 0.5 M mannitol and 1% activated
charcoal (treatment IV).