The average motility scores of WAD buck
spermatozoa in 5 trials were 52.6%, 19.6% and
9.0% in D2, D3 and D4, respectively, at 2 hours
post-extension (p.e.) of semen. The same
parameter in the 5 trials at 3 hours p.e was 45%,
15% and 5.6%, respectively, in D2, D3 and D4.
These results showed that survival of WAD
buck sperm cells is inversely related to the
concentration of coconut milk as the motility
scores decreased from D2 to D4 at both 2 and 3
hours storage time. The reason for this
observation could be that the increasing quantity
of coconut milk increased the viscosity of the
extenders, which thereby hindered spermatozoal
progressive movement. In comparing different
proportions of extenders, statistical analysis
showed that after 2 hours storage of extended
semen at room temperature, extender D2
preserved a mean motility score of 52.6% which
was significantly (p = 0.018) higher than mean
scores observed in D3 and D4. Also at 3 hours
p.e., D2 that maintained sperm cells at average
motility score of 45% was superior (p = 0.012)
to D3 and D4. Comparison of storage time
following semen extension revealed that ability
of D2 at preserving sperm cell motility at 2 and
3 hours p.e. was not statistically different (p =
0.693). This result was suggestive of possibility
of using D2 as WAD buck semen extender for
duration of 3 hours at room temperature. The
observation was the same when mean sperm cell
motility scores at 2 and 3 hours p.e. were
compared (p = 0.609) in D3.
These results of storage time effect on extended
semen was somehow not in consonance with