Half of the amount of the vegetable and of the medium in
the jar were taken aseptically followed by a quick freezing in
liquid nitrogen, while the remaining vegetable and medium
of the sample was mixed thoroughly with a stomacher for
further microbiological analysis. The raw, withered, and fermented
vegetables as well as the fermentation medium were
frozen in liquid nitrogen followed by lyophilisation. Subsequently,
the vegetables were grinded and stored at −22 °C
until further analyses of GLS and myrosinase activity. These
analyses were also performed to the fermentation medium.