3. Results
3.1. EST-SSR amplification of 129 spring orchid cultivars
A total of 120 alleles were generated by the 13 EST-SSR markers with an average of 9 per marker. A set of 111 out of the 120 alleles were polymorphic (92.50%), among the 129 spring orchid cultivars (Table 2). The number of alleles per primer pair ranged from 5 (CY17) to 14 (CY34) (Table 2).
3.2. Genetic diversity among seven horticultural types of C. goeringii cultivars
Genetic diversities of 120 spring orchid cultivars with typical horticultural types were analyzed with the 13 EST-SSR markers. Among the 111 polymorphic alleles amplified, the plum petal type contributed the most alleles (102, 85.00%), whereas the colored flower type contributed the least (38, 31.67%) (Table 3). The average number of polymorphic alleles among these seven horticultural types was 73 alleles per type (Table 3).
Table 3.
Genetic diversity of seven horticultural types of C. goeringii.