Maximum activities for the recombinant AmyA and GlaA in S. cerevisiae Y294 were observed at pH 4.0 and pH 4.5, respectively, with significant activity detected for both enzymes at pH 3 to 5 (Figure 3). These maxima compared well with those reported for other Aspergillus α-amylase and glucoamylases [3,16,29] and are aligned with the growth conditions of S. cerevisiae, which is desirable for a consolidated process [30]. The temperature maxima (Figure 3) for the recombinant AmyA (60°C) and GlaA (70°C) were slightly higher than the 40 to 60°C generally reported for raw starch degrading α-amylases and glucoamylases [3,16,31].