1. Label 50ml polypropylene screw-cap or 50ml Oak Ridge tubes with culture numbers.
2. Prepare Cell Lysis Buffer (50 mM Tris:50 mM EDTA, pH 8.0 + 1% Sarcosyl) as follows:
2.1. 50 ml of 1 M Tris, pH 8.0
2.2. 100 ml of 0.5 M EDTA, pH 8.0
2.3. 100 ml of 10 % Sarcosyl (N-Lauroylsarcosine, Sodium salt)
2.4. Dilute to 1000 ml with sterile Ultrapure water (CLRW)
3. Calculate the total volume of Cell Lysis/Proteinase K Buffer needed as follows:
3.1. 5 ml Cell Lysis Buffer is needed per tube
e. g., 5 ml x 10 tubes = 50 ml
3.2. 25 µl Proteinase K stock solution (20 mg/ml) is needed per tube of the cell lysis buffer
e. g., 25 µl x 10 tubes = 250 µl
1. Label 50ml polypropylene screw-cap or 50ml Oak Ridge tubes with culture numbers.2. Prepare Cell Lysis Buffer (50 mM Tris:50 mM EDTA, pH 8.0 + 1% Sarcosyl) as follows:2.1. 50 ml of 1 M Tris, pH 8.02.2. 100 ml of 0.5 M EDTA, pH 8.02.3. 100 ml of 10 % Sarcosyl (N-Lauroylsarcosine, Sodium salt)2.4. Dilute to 1000 ml with sterile Ultrapure water (CLRW)3. Calculate the total volume of Cell Lysis/Proteinase K Buffer needed as follows:3.1. 5 ml Cell Lysis Buffer is needed per tubee. g., 5 ml x 10 tubes = 50 ml3.2. 25 µl Proteinase K stock solution (20 mg/ml) is needed per tube of the cell lysis buffere. g., 25 µl x 10 tubes = 250 µl
การแปล กรุณารอสักครู่..