Carbohydrates, which play key roles as structural and
energy compounds in cells, are distinguished as
monosaccharide and disaccharide. Various carbon sources
such as glucose, fructose, maltose, sucrose, and lactose, were
tested, respectively, to find the optimal medium for
ribonuclease production. For the maximal production of
ribonuclease from Aspergillus niger ATCC26550, glucose
was found to be the most effective carbon source of all the
different carbon sources used glucose was found to be the
most suitable to induce the ribonuclease production. The
carbon content was maintained constant in all experiments.
Fig. 1 shows the different carbon sources (3%) studies in the
production medium of ribonuclease enzyme. To optimize the
nitrogen content various sources of nitrogen were used as
shown in Fig. 2. The nitrogen level was maintained the same
in all cases as 88.75mg of nitrogen/50ml of media. It was
found that beef extract and peptone gave the best results and
was chosen as the suitable nitrogen source for ribonuclease
production. The maximum enzyme production was studied at
pH range of 3.0-6.0. Optimum pH and temperature for
enzyme production was found to be 5.5 and 30°C
respectively(Fig.3). Maximum production was at around 5
days (120hr) of cultivation.