Microporous, non-woven poly(e-caprolactone) (PCL) scaffolds were made by electrostatic fiber spinning. In this process, polymer
fibers with diameters down to the nanometer range, or nanofibers, are formed by subjecting a fluid jet to a high electric field.
Mesenchymal stem cells (MSCs) derived from the bone marrow of neonatal rats were cultured, expanded and seeded on electrospun
PCL scaffolds. The cell-polymer constructs were cultured with osteogenic supplements under dynamic culture conditions for up to 4
weeks. The cell-polymer constructs maintained the size and shape of the original scaffolds. Scanning electron microscopy (SEM),
histological and immunohistochemical examinations were performed. Penetration of cells and abundant extracellular matrix were
observed in the cell-polymer constructs after 1 week. SEM showed that the surfaces of the cell-polymer constructs were covered with
cell multilayers at 4 weeks. In addition, mineralization and type I collagen were observed at 4 weeks. This suggests that electrospun
PCL is a potential candidate scaffold for bone tissue engineering.
Microporous, non-woven poly(e-caprolactone) (PCL) scaffolds were made by electrostatic fiber spinning. In this process, polymer
fibers with diameters down to the nanometer range, or nanofibers, are formed by subjecting a fluid jet to a high electric field.
Mesenchymal stem cells (MSCs) derived from the bone marrow of neonatal rats were cultured, expanded and seeded on electrospun
PCL scaffolds. The cell-polymer constructs were cultured with osteogenic supplements under dynamic culture conditions for up to 4
weeks. The cell-polymer constructs maintained the size and shape of the original scaffolds. Scanning electron microscopy (SEM),
histological and immunohistochemical examinations were performed. Penetration of cells and abundant extracellular matrix were
observed in the cell-polymer constructs after 1 week. SEM showed that the surfaces of the cell-polymer constructs were covered with
cell multilayers at 4 weeks. In addition, mineralization and type I collagen were observed at 4 weeks. This suggests that electrospun
PCL is a potential candidate scaffold for bone tissue engineering.
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