A. polyanthum extract, compounds 9, 4 and 5 showed selective activities, the IC50 values <40 μg/mL being obtained on 5/9, 4/9, 2/9 and 1/9 tested cell lines respectively (Table 1). According to the National Cancer Institute (USA), 30 μg/mL is the upper IC50 limit considered promising for purification of a crude extract [34]. We therefore, tested a slightly higher concentration of 40 μg/mL in our preliminary assay. Also, the IC50 threshold value of 4 μg/ml or 10 μM [35,36] after 48 and 72 h incubations has been set to identify good cytotoxic compounds. Considering these thresholds, the IC50 values below or around 30 μg/mL were recorded with A. arundinaceum extract against eight of the nine tested cancer cell lines (Table 1) explaining why it was considered further for purification. Nonetheless, the extract from A. polyanthum also showed activities with IC50 values <30 μg/mL on four of the nine tested cancer cell lines.
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