Experiments were performed in a 3L bioreactor with 1.5 L
working volume. Millipore guava easyCyte 5HTTM flow
cytometer with guava ViaCountTM was used for cell density
and viability measurements. MultiCaspaseTM for caspase
activity-late apoptosis, NexinTM for annexin binding-early
apoptosis and Cell CycleTM assays were performed. FlowJo
version 10 (FLOWJO LLC) was used to fit the cell cycle
populations. Glucose, lactate, and sodium measurements
were performed using Nova Biomedical FLEX an average of
three times a day.