Liver tissue samples were taken and homogenized with a mixer. Three grams of homogenized liver tissue samples were taken and mixed with chloroform-methanol (2:1,v/v) in a mixer. The homogenate was centrifuged at 5000 rpm for 5 min at 4°C and the supernatant part was used in the ADEK vitamin and fatty acid analysis [23]. Nonlipid contaminants in lipid extracts
were removed by 0.88% KCl solution. The extracts were evaporated in rotary evaporator flask and then stored at -25°C.