Spodoptera litura egg masses were collected from groundnut fields at Vellore and Kancheepuram districts of Tamil Nadu, India. After egg hatching, castor (Ricinus communis) leaves were provided for larval feeding till the pupal stage under laboratory condition (28 ±2º C and 80 ±5% R.H.). Sterilized soil was provided for pupation. After pupation, the pupae were collected from the soil and placed inside cage for adult emergence. Ten per cent honey solution mixed with few drops of multi-vitamin was provided for adult feeding to increase the rate of fecundity. Folded filter papers were provided for egg laying. After egg
laying, egg masses were collected from the filter paper and was allowed for hatching. This process of culture method was repeated and the culture was maintained throughout the study period.