Therefore, in this study, we aimed to identify the original
feed source naturally contaminated by ZEN in one
of the studied cattle herds. To this end, we used a ZEN
ELISA kit and confi rmed the results using HPLC. In addition,
the effects of intervention against contamination
by feeding a ZEN noncontaminated diet to the herd were
evaluated by monitoring urinary ZEN concentrations.
(Avantaggiato et al., 2007; Binder et al., 2007). In farm
animals, the concentration of ZEN and its metabolites
in urine may be used as potential biomarkers of ZEN
exposure