Transformed tobacco calli were selected on 40-mg/L Ignite
and subculrured three times on herbicide-supplemented Murashige-
Skoog plates. The transformed transgenic calli had a
normal appearance and had similar growth characteristics as
the control calli. Southern blot analysis of Hmdlll-digested
genomic DNA from several independently transformed
transgenic calli showed the expected 2.4-kb bands (Fig. 1,
bottom left). All herbicide-resistant calli tested had this band.
Northern blot analysis of the same calli indicated the presence
of 2.3-kb transcripts (Fig. 1, bottom right).