Silk fibroin (SF), is a very attractive protein-polymer be processed into a variety of formats to
match structural and morphological features for specific biomedical applications. The aim of
the present work is to investigate blood compatibility of two forms, films and scaffolds, of
silk fibroin- N, N’ methylene bisacrylamide (MBA) prepared by using blend solutions of the
two components. Biofilms were prepared under UV-irradiation while scaffolds were prepared
via freeze-drying technique at -30oC and -80oC, respectively. Swelling, biodegradation tests
with protease enzyme, FTIR, SEM, XRD analyses were applied to characterize the
biomaterials. The results indicated that, the presence of the crosslinker (MBA) in the scaffold
and biofilm aids the formation of ordered structure. The pore size and biodegradability can be
controllable by the amount of crosslinker. The anticoagulant activity was evaluated using
prothrombin time (PT), activated partial thromboplastin time (APTT). The in-vitro
coagulation test and platelet adhesion test analyses indicated that the modified scaffolds and
biofilms exhibited better hemocompatibility in comparison with pure silk fibroin.
These results demostrated that the silk fibrion –N, N’ methylene bisacrylamide biofilms and
blended scaffolds have potential applications as blood contact device.