2.2. Extraction of antifungal compounds from D. metel
Antifungal activity of D. metel was demonstrated by various
authors (Kagale et al., 2004; Kishore and Pandey, 2005). Leaves of
D. metel were homogenized in different solvents (1:1 w/v) viz.,
methanol, acetone, diethyl ether, ethyl acetate and chloroform separately.
Extracts were filtered through two layers of muslin cloth
and centrifuged at 7500g for 20 min. Solvent phase of the extracts
were evaporated in vacuum at 40 C using a Rotavapor R-114 (Bucchi)
and the residue obtained was dissolved in 2 ml of sterile distilled
water. The antifungal nature of various solvent extracts
was tested against the mycelial growth of Foc by inhibition zone
technique (Bauer et al., 1966).
2.2. Extraction of antifungal compounds from D. metelAntifungal activity of D. metel was demonstrated by variousauthors (Kagale et al., 2004; Kishore and Pandey, 2005). Leaves ofD. metel were homogenized in different solvents (1:1 w/v) viz.,methanol, acetone, diethyl ether, ethyl acetate and chloroform separately.Extracts were filtered through two layers of muslin clothand centrifuged at 7500g for 20 min. Solvent phase of the extractswere evaporated in vacuum at 40 C using a Rotavapor R-114 (Bucchi)and the residue obtained was dissolved in 2 ml of sterile distilledwater. The antifungal nature of various solvent extractswas tested against the mycelial growth of Foc by inhibition zonetechnique (Bauer et al., 1966).
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